abstract：:We have shown previously that the copy number of plasmid pSY10 from the marine cyanobacterium Synechococcus sp. NKBG 042902 is dependent on the salinity of the growth medium. We report here the complete nucleotide sequence (2561 bp) of this plasmid. The longest open reading frame, ORF-B (1.08 kb), occurs on a 1.6-kb E...

journal_title：Plasmid

authors： Kawaguchi R,Nagaoka T,Burgess JG,Takeyama H,Matsunaga T

更新日期：1994-11-01 00:00:00

abstract：:A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimat...

journal_title：Plasmid

authors： Coronado C,Vázquez ME,Cebolla A,Palomares AJ

更新日期：1994-11-01 00:00:00

abstract：:During bacterial infection, pathogens are exposed to a variety of stimuli, e.g., sudden temperature increase on entering mammalian host or oxidative stress associated with exposure to phagocytes. Yersinia enterocolitica, which is a facultative intracellular bacteria, responds to macrophage phagocytosis by the producti...

journal_title：Plasmid

authors： Yamamoto T,Hanawa T,Murayama SY,Ogata S

更新日期：1994-09-01 00:00:00

abstract：:Little knowledge of DNA replication in cyanobacteria is available. In this study, we report the development and characterization of an in vitro system for studies of replication of the endogenous plasmids from the unicellular cyanobacterium Synechocystis 6803. This system (fraction III) was isolated at high salt conce...

journal_title：Plasmid

authors： Yang X,Daniell H,McFadden B

更新日期：1994-09-01 00:00:00

abstract：:Heterologous expression in Escherichia coli is often limited by yield and solubility of the foreign protein in the bacterial cytoplasm. In many cases, overexpression also results in growth inhibition. In order to produce retroviral proteins that are especially difficult to overexpress in E. coli, we designed a set of ...

journal_title：Plasmid

authors： Valverde V,Duplan H,Knibiehler M,Masson JM

更新日期：1994-07-01 00:00:00

abstract：:Avirulence gene D (avrD) in strain PT23 of Pseudomonas syringae pv. tomato (Pst) specifies the production of syringolides, which are elicitors of plant defense reactions. An 83-kb indigenous plasmid (pPT23B) that carries avrD has been mapped and characterized and a putative par region was identified. pPT23B contains a...

journal_title：Plasmid

authors： Murillo J,Shen H,Gerhold D,Sharma A,Cooksey DA,Keen NT

更新日期：1994-05-01 00:00:00

abstract：:Dictyostelium mucoroides plasmids Dmp1 and Dmp2 are naturally occurring compatible members of the Dictyostelium Ddp1 plasmid family found in the same wild isolate strain. The nucleotide sequences of Dmp1 (5983 bp) and Dmp2 (6018 bp) are 74% identical and each carries open reading frames (ORFs) similar to the G1 and G5...

journal_title：Plasmid

authors： Kiyosawa H,Hughes JE,Welker DL

更新日期：1994-03-01 00:00:00

abstract：:Sequence analysis of the Pseudomonas aeruginosa insertion sequence element IS222 revealed it to be 1234 bp in size with 23 bp imperfect terminal inverted repeats. Insertion caused a 5-bp duplication of the insertion site. Two ORFs were identified, one of which, ORFA, could encode a basic (pI 10.5) polypeptide with a m...

journal_title：Plasmid

authors： Kropinski AM,Farinha MA,Jansons I

更新日期：1994-03-01 00:00:00

abstract：:pIP501 and pAM beta 1 are promiscuous plasmids from gram-positive bacteria which carry structurally and genetically related replication functions (L. Jannière, A. Gruss and S.D. Ehrlich, 1993, in "Bacillus subtilis and Other Gram-positive Bacteria: Biochemistry, Physiology, and Molecular Genetics (A.L. Sonenshein, J.A...

journal_title：Plasmid

authors： Pujol C,Ehrlich SD,Jannière L

更新日期：1994-01-01 00:00:00

abstract：:The imidazole and triazole fungicides inhibit cytochrome P450 14 alpha-lanosterol demethylase (P45014DM) implicated in the ergosterol biosynthesis pathway, which is specific to fungi and yeasts. Two plasmids were obtained which allow triazole and imidazole resistance in Saccharomyces cerevisiae. The low copy number pl...

journal_title：Plasmid

authors： Doignon F,Aigle M,Ribereau-Gayon P

更新日期：1993-11-01 00:00:00

abstract：:We present the complete nucleotide sequence of a mer operon located on a 60-kb conjugative plasmid pKLH2 from an environmental bacterium, Acinetobacter calcoaceticus, isolated from a mercury mine. The pKLH2 mer operon has essentially the same gene organization as that of Tn21 and Tn501 from clinical bacteria. The pKLH...

journal_title：Plasmid

authors： Kholodii GY,Gorlenko Z,Lomovskaya OL,Mindlin SZ,Yurieva OV,Nikiforov VG

更新日期：1993-11-01 00:00:00

abstract：:The corrected DNA sequence of the tet(K) structural gene, encoding the tetracycline efflux protein from Staphylococcus aureus, is reported. The Tet(K) protein is homologous to related tetracycline efflux proteins throughout the protein, including at the C-terminal end. Hydropathy plotting now clearly indicates that th...

journal_title：Plasmid

authors： Guay GG,Khan SA,Rothstein DM

更新日期：1993-09-01 00:00:00

abstract：:Escherichia coli strain K12 BM14, carrying plasmid R751 (51.4 kb; Tpr, Tra+, IncP), was used as donor in matings with reference strains of the six Brucella nomenspecies, not known so far to harbor naturally occurring plasmids. R751 was easily transferred to and between Brucella spp., at frequencies ranging between 10(...

journal_title：Plasmid

authors： Verger JM,Grayon M,Chaslus-Dancla E,Meurisse M,Lafont JP

更新日期：1993-03-01 00:00:00

abstract：:The complete nucleotide sequence of a plasmid, designated pRJF1, isolated from a rumen bacterium of the genus Butyrivibrio, has been determined. pRJF1 is a small plasmid (2631 bp) which shows the high AT content (64%) typical of plasmids from gram-positive organisms. Computer analysis of sequence data revealed two maj...

journal_title：Plasmid

authors： Hefford MA,Teather RM,Forster RJ

更新日期：1993-01-01 00:00:00

abstract：:Transcription of the 7.5-kb cryptic plasmid of Chlamydia trachomatis serovar L2 was investigated. Faint, diffuse transcripts of about 1.6 and 2.2 kb and intense short transcripts of about 250 and 430 bases were identified by Northern blot analysis. The short transcripts were found to have a common 5' end corresponding...

journal_title：Plasmid

authors： Fahr MJ,Sriprakash KS,Hatch TP

更新日期：1992-11-01 00:00:00

abstract：:A plasmid shuttle vector for Escherichia coli and mycobacteria was constructed from an E. coli plasmid containing the ColE1 origin, a 2.6-kb PstI fragment from bacteriophage D29 that grows in numerous mycobacterial species, and the kanamycin resistance gene either of Tn903 or of Tn5. The resultant plasmid is 7.63 kb a...

journal_title：Plasmid

authors： David M,Lubinsky-Mink S,Ben-Zvi A,Ulitzur S,Kuhn J,Suissa M

更新日期：1992-11-01 00:00:00

abstract：:Two improved Deinococcus radiodurans-Escherichia coli shuttle vectors have been constructed. pI3 is a 16-kb plasmid that confers chloramphenicol resistance in D. radiodurans (CmR, cat) and ampicillin resistance in E. coli (ApR) and contains a multiple cloning site that does not interrupt sequences necessary for replic...

journal_title：Plasmid

authors： Masters CI,Minton KW

更新日期：1992-11-01 00:00:00

abstract：:The 4.2-kb cryptic plasmid pVA380-1 has been used as a vector for the cloning of antibiotic resistance genes directly in streptococci, and in the construction of Escherichia coli/Streptococcus shuttle vectors. The results of subcloning experiments located the basic replicon of pVA380-1 within a 2.5-kb region. The nucl...

journal_title：Plasmid

authors： LeBlanc DJ,Lee LN,Abu-Al-Jaibat A

更新日期：1992-09-01 00:00:00

abstract：:The chimeric plasmid pBN183 was first constructed in Escherichia coli by ligating the BamHI-digested E. coli plasmid pBR322 and a Bg/II-linearized streptococcal plasmid, pNZ18. The pBN183 transformed E. coli to ApR at a frequency of (8.2 +/- 1.2) x 10(5) colony forming units (CFU)/microgram DNA. Electrotransformation ...

journal_title：Plasmid

authors： Solaiman DK,Somkuti GA,Steinberg DH

更新日期：1992-07-01 00:00:00

abstract：:We have cloned and characterized an insertion sequence from Leuconostoc mesenteroides subsp. cremoris strain DB1165. This element, designated IS1165, is 1553 bp, has imperfect inverted repeat ends, contains an open reading frame of 1236 bp, and is not related to any previously described insertion sequence. The copy nu...

journal_title：Plasmid

authors： Johansen E,Kibenich A

更新日期：1992-05-01 00:00:00

abstract：:A new plasmid, pNQ116, was constructed in Bacillus sphaericus by cloning a promoter fragment from B. sphaericus Ts-1 into pNQ112. The plasmid (CmrKmr, 5.23 kb) contains a restriction endonuclease polylinker used for cloning foreign genes, and its cat-86 gene is expressed at high levels from the Ts-1 promoter. This pla...

journal_title：Plasmid

authors： Qiao M,Gao C,Cai B,Zhang X,Wang S

更新日期：1992-05-01 00:00:00

abstract：:The ability of some bacteria to grow in the presence of high concentrations of tellurium compounds has been recognized for almost 100 years. Since then, interest in this phenomenon has generated a slow but steady trickle of literature. In the past few years, the use of modern techniques in molecular biology has led to...

journal_title：Plasmid

authors： Walter EG,Taylor DE

更新日期：1992-01-01 00:00:00

abstract：:pBR322-derived plasmids that lack the bla gene and 40% of the gene for the replication inhibitor, RNAI, have been constructed. Since the RNAI gene totally overlaps with the gene for the replication primer, RNAII, this primer is similarly defective and also lacks its normal promoter. The primer is presumed to by synthe...

journal_title：Plasmid

authors： Chiang CS,Bremer H

更新日期：1991-11-01 00:00:00

abstract：:Two independently isolated temperature-sensitive bacteriophage that are specific for enterobacterial hosts harboring HI and HII plasmids were characterized to determine if any identifiable differences existed between them. The traits examined included adsorption pattern of phage to H pili, bacteriophage size, sensitiv...

journal_title：Plasmid

authors： Maher D,Sherburne R,Taylor DE

更新日期：1991-09-01 00:00:00

abstract：:A phagemid vector library for cloning DNA with four nucleotide 5' or 3' overhangs has been constructed. This library is based on the pT7T3 vector (Pharmacia) which is a modification of the phagemid pTZ18U vector. We have chosen pT7T3 as the parent vector because it can be used for Sanger's dideoxy sequencing and for t...

journal_title：Plasmid

authors： Waye MM,Mui F,Hodge K,Li VK

更新日期：1991-07-01 00:00:00

abstract：:Lactococcus lactis ME2 can transfer a 46-kb plasmid, pTR2030, which encodes abortive phage infection (Hsp) and restriction/modification (R/M) activities. pTR2030 can be detected as a monomeric plasmid in transconjugants at low copy number, but not in ME2. pTR2030-specific probes were cloned and used to determine the l...

journal_title：Plasmid

authors： Hill C,Miller LA,Klaenhammer TR

更新日期：1991-03-01 00:00:00

abstract：:The structure of several T-DNAs of Agrobacterium tumefaciens was determined by molecular cloning and Southern hybridization. The T-DNAs cloned in Escherichia coli vectors from four different nopaline type strains (PyTE1, PO31, PO22, and AKE10) showed various sizes of restriction enzyme fragments. Comparative analysis ...

journal_title：Plasmid

authors： Wabiko H,Kagaya M,Sano H

更新日期：1991-01-01 00:00:00

abstract：:In order to select insertion sequences able to promote transcription of flanking genes (ISp elements), three mobilizable RSF1010 derived vectors were constructed. Using promoterless antibiotic resistance genes, ISp elements ranging from 0.75 to 2.9 kb were isolated from Escherichia coli and Rhizobium meliloti. Restric...

journal_title：Plasmid

authors： Labes G,Simon R

更新日期：1990-11-01 00:00:00

abstract：:A number of plasmid vectors were constructed for high-level gene expression in the dimorphic gram-negative bacterium Caulobacter crescentus. These vectors incorporate the transcription and translation initiation regions of the C. crescentus CB15A rsaA gene, which codes for the abundantly synthesized protein comprising...

journal_title：Plasmid

authors： Bingle WH,Smit J

更新日期：1990-09-01 00:00:00

abstract：:Here we present evidence that the cryptic 5.6-kb plasmid found in certain strains of Streptococcus mutans is not involved in mutacin production. This evidence comes from demonstrating similarities between a plasmid-less strain T8 and a group II plasmid strain UA96. Both produce what appears to be an identical mutacin ...

journal_title：Plasmid

authors： Caufield PW,Shah G,Hollingshead SK,Parrot M,Lavoie MC

更新日期：1990-09-01 00:00:00

abstract：:A flexible M13 vector library incorporating the BstXI site has been developed. DNA cut by any currently commercially available restriction endonuclease that generates a 4-nucleotide (nt) 3' overhang can be ligated into a specific clone of the library. The BstXI enzyme recognizes a 6-bp bipartite palindromic sequence. ...

journal_title：Plasmid

authors： Panchmatia FK,Cramb EM,Li V,Allen FL,Glickman BW,Waye MM

更新日期：1990-07-01 00:00:00

abstract：:From Enterococcus faecalis cells containing random chromosomal insertions of Tn916, strains resistant to a lytic phage were selected and tested for conjugal mating ability. The phage-resistant strains all showed decreased recipient ability (Con-) in broth matings with donors carrying pheromone-inducible plasmids. Thes...

journal_title：Plasmid

authors： Trotter KM,Dunny GM

更新日期：1990-07-01 00:00:00

abstract：:Homology was previously detected between the DNA restriction fragments containing Rhizobium meliloti nodulation genes and the 90-MDa plasmid, p90, of Azospirillum brasilense Sp7. Two DNA loci from Sp7 genome that complement mutations in the exopolysaccharide synthesis genes, exoB and exoC, of R. meliloti were also sho...

journal_title：Plasmid

authors： Onyeocha I,Vieille C,Zimmer W,Baca BE,Flores M,Palacios R,Elmerich C

更新日期：1990-05-01 00:00:00

abstract：:Since reduced metronidazole causes DNA damage, resistance to metronidazole was used as a selection method for the cloning of Bacteroides fragilis genes affecting DNA repair mechanisms in Escherichia coli. Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli AB1157 and uvrA, B, and...

journal_title：Plasmid

authors： Wehnert GU,Abratt VR,Goodman HJ,Woods DR

更新日期：1990-03-01 00:00:00

abstract：:Plasmid pT181 is a small multicopy plasmid from Staphylococcus aureus that belongs to incompatibility group 3 and expresses two distinct types of incompatibility, Inc3A and Inc3B. Inc3A incompatibility is expressed by the primary replication control determinant, copA, which specifies two small transcripts, RNA I and R...

journal_title：Plasmid

authors： Highlander SK,Novick RP

更新日期：1990-01-01 00:00:00

abstract：:Four plasmids encoding restriction and modification (R/M) systems are described that are different in the specificity of their restrictive activity toward the small isometric phage p2 and prolate phage c2. The R/M plasmids were cotransformed into Lactococcus lactis MG1363 with pVS2, encoding resistance to chlorampheni...

journal_title：Plasmid

authors： Josephsen J,Klaenhammer T

更新日期：1990-01-01 00:00:00

abstract：:Tn4527 was isolated from a Salmonella typhimurium strain obtained in Brazil. Its size is 19.6 kb and it carries resistance to trimethoprim, spectinomycin, and streptomycin, as in the case of Tn7 (14 kb). A restriction analysis of the transposon shows regions of similarity to Tn7 mixed with extra DNA. The 2.6-kb and 2....

journal_title：Plasmid

authors： Dumans AT,Carvalho MF,Coelho A

更新日期：1989-11-01 00:00:00

abstract：:The CoIV plasmids are large virulence plasmids of the incompatibility group IncFI. We have obtained the genetic maps of two of the most studied CoIV plasmids, pCoIV-B188 and pCoIV-K30, according to restriction enzyme sites and landmark phenotypic characteristics such as colicin V, the aerobactin iron uptake system, th...

journal_title：Plasmid

authors： Waters VL,Perez-Casal JF,Crosa JH

更新日期：1989-11-01 00:00:00

abstract：:The joining of DNA ends was compared in the established CV1 cell line and in African green monkey kidney primary cells, from which the CV1 cell line was established. Using a linear SV40 genome that carries a terminal repeat of 526 nucleotide pairs, we measured the efficiency of circularization by end joining relative ...

journal_title：Plasmid

authors： Zheng H,Chang XB,Wilson JH

更新日期：1989-09-01 00:00:00

abstract：:We have evaluated the ability of immortalized human fibroblasts to recombine transfected plasmid DNA. A number of cell lines from normal individuals and from patients with DNA damage-processing defects were examined. Two plasmid recombination substrates were derived from pSV2neo and contained nonoverlapping deletions ...

journal_title：Plasmid

authors： Timme TL,Wood CM,Moses RE

更新日期：1989-07-01 00:00:00